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Hydrogen gas inhibits lung cancer progression through targeting SMC3

  • Time:2018年
  • Journal:Biomedicine & Pharmacotherapy
  • Disease Model:Lung cancer cell lines
  • Corresponding Author:Dongchang Wang, Lifei Wang, Yu Zhang, Yunxia Zhao, Gang Chen⁎
  • Corresponding Unit:未录入
  • Experimental Subject:BALB/c mices
  • Way of Hydrogen Administration:Inhalation of Hydrogen
  • Dosage:hydrogen gas concentration (20%, 40%, 60%, (vol/vol))(in );67% H2- 33%O2 (In vivo)
  • Duration:66.6% H2 2 h every day, for 4 weeks
Main Result

H2 inhibited cell viability, migration and invasion, and catalyzed cell apoptosis and H2 induced A549 and H1975 cells G2/M arrest. Besides, H2 down-regulated the expression of NIBPL, SMC3, SMC5 and SMC6, and also reduced the expression of Cyclin D1, CDK4 and CDK6. H2 translocated the subcellular location of SMC3 during cell division and decreased its stability and increased its ubiquitination in both A549 and H1975 cells. In addition, inhibition of the proliferation, migration and invasion and promotion of the apoptosis of A549 and H1975 cells induced by H2 were all abolished when overexpressed SMC3 in the presence of H2. Animal experimental assay demonstrated that the tumor weight in H2 group was significantly smaller than that in control group, but was bigger than cis-platinum group. The expression of Ki-67, VEGF and SMC3 were decreased when mice were treated with H2 or cis-platinum, especially for cis-platinum. All data suggested that H2 inhibited lung cancer progression through down-regulating SMC3, a regulator for chromosome condensation, which provided a new method for the treatment of lung cancer.

Classification of Disease:Spinal cord and nerve system disease